NEXTFLEX 16S V3-V4The NEXTFLEX 16S V3 V4 Amplicon Seq Library Prep Kit is designed for the preparation of multiplexed amplicon libraries that span the hypervariable domains three through four (V3 and V4) of microbial 16S ribosomal RNA (rRNA) genes. These libraries are compatible with paired end sequencing on the Illumina and Element Biosciences sequencing platforms. There are two main steps involved in 16S V3 V4 amplicon processing: an initial PCR amplification using