AmpFi cDNA Synthesis KitcDNA synthesis performance using AmpFi Reverse Transcriptase remains high in the presence of various inhibitors. lsopropanol, BugBuster,or Heparin inhibitors were added in different concentrations to an RNA sample prior to cDNA synthesis using AmpFi Reverse Transcriptase. After synthesis, 0. 5 3 kb targets were amplified through PCR and visualized with a 1% agarose gel. Resistance to isopropanol was maintained up to 5% while resistance to BugBuster